single cell RNA analysis

Question

[archanaverma433]

I have scRNA data, but I have sample biasness. Some samples have only one type of cells. how to deal with this situation and go further. Also Can anyone help me in how to apply scaling factor like I have to apply different scaling factor on different samples or apply scaling factor on groupwise?

[rpolicastro]

Can you provide more information on your samples, and what you're trying to accomplish?

[archanaverma433]

I have PBMC cells of normal and tumor samples. and in some samples there is only one type of PBMC cells example - only lymphoid cells. How to proceed with this data and also some samples have more counts and some have very less counts then how to use scaling factor, do we have to apply different scale factor on individual samples or only one scaling factor for all samples?