Deleted:Trimmomatic and bwa-mem
1
0
Entering edit mode
24 months ago
anasjamshed ▴ 140

I uploaded my NGS data files in Galaxy and then ran fastqc to check the quality of both files. According to fastqc report, fastq files contain adapter sequences that I need to remove so I used Trimmomatic. But Trimmomatic produced 4 files:R1 paired , R2 paired , R1 unpaired and R2 unpaired. Which of these 2 files I can use in bwa-mem to align with the reference genome?

alignment Galaxy • 609 views
ADD COMMENT
This thread is not open. No new answers may be added
Traffic: 2745 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6