Entering edit mode
24 months ago
anasjamshed
▴
140
I uploaded my NGS data files in Galaxy and then ran fastqc to check the quality of both files. According to fastqc report, fastq files contain adapter sequences that I need to remove so I used Trimmomatic. But Trimmomatic produced 4 files:R1 paired , R2 paired , R1 unpaired and R2 unpaired. Which of these 2 files I can use in bwa-mem to align with the reference genome?