Entering edit mode
19 months ago
csag6433
•
0
Hi. i blasted several short nucleotide sequences that are around 45bp long and very similiar in sequence to each other against a bacterial genome. now i got many overlapping hits for the same region in the bacterial genome. i would like to keep just the best hit for a specific region by bitscore or evalue. so whenever hits share atleast 1bp in positon just keep the best hit remove all others. how can i do that on a big blast output ? i didnt succeed so far
have a nice day
heres a short example of my output . as you can see there are many overlapping hits per subject sequence from different queries . i would like to keep only the best hit per subject region independent of the type of query