Error while running GATK4 Mutect2 in Galaxy

Question

[anasjamshed]

I have one clown and one control sample of python molurus bivittatus. After alignment, I am trying to use GATK4 Mutect2 to joint variant calling:

But after executing I got this error:

A USER ERROR has occurred: Bad input: The given bam input has no sample names.

How can I solve it?

Answer 1

[Pierre Lindenbaum]

bam is missing read groups: https://gatk.broadinstitute.org/hc/en-us/articles/360035890671-Read-groups

[anasjamshed]

so how can i add read groups?

[Pierre Lindenbaum]

Adding Read Groups To Bam Files