I'm trying to download a lot of SRA files which are whole genome of animals. average sizes are tens of gigabytes so it takes almost a day to download one individual even if I use SRAtoolkit prefetch and than fasterq-dump. I know there are other ways like wget from FTP but FTP addresses change often and even if I use something like SRA explorer, it, doesn't receive multiple SRR so I have to do it manually. Also wget from FTP is too slow also. I'm assuming that ascp of Aspera is the fastest way, but I've been struggling to set it. I tried the aspera connect version 4.2.5 but it doesn't give me keys after installing, so I tried with lower versions.
ascp -QT -l 300m -P33001 -i $HOME/.aspera/connect/etc/asperaweb_id_dsa.openssh era-fasp@fasp.sra.ebi.ac.uk:vol1/fastq/ERR315/009/ERR3155119/ERR3155119.fastq.gz ./
This is the command I tried cause people said it worked about 5 months ago. but it gave me Session Stop (Error: Server aborted session: No such file or directory) error as if the address is wrong.
I checked in the SRA explorer for the ascp address but even if I use the code given by SRA explorer, it doesn't work. Is there someone who uses ascp for SRA downloading? could you please help me
Thanks but it still doesn't work does it work for you?
What does not work?
http://ftp.sra.ebi.ac.uk/vol1/fastq/ERR315/009/ERR3155119/ERR3155119.fastq.gz
is a valid file URL. I don't have a way to test with
ascpnow.even if I add the / in my command, it gives the same error I've checked that the ftp adress is valid but I'm not sure about this host?source? adress era-fasp@fasp.sra.ebi.ac.uk:vol1/fastq/ERR315/009/ERR3155119/ERR3155119.fastq.gz It doesn't work for me so I'm wondering if it's my network problem or the address problem I contacted IBM but they won't give me answers cause it's a free program