The web app at http://www.microbioma16s.it allows user-friendly interactive analysis of microbiome related NGS data from meta-barcoding experiments of the 16S rRNA gene, with a UI easy to work out. It allows both pre-processing of raw reads (fastq.gz files from Illumina MiSeq technology) and direct processing of OTU and taxonomy tables along with associated samples' metadata to obtain alpha and beta diversity metrics as well as taxa relative abundances. All results (figures and tables) are available for download. Written in Italian and English.