'Seurat' package

Question

[sooni]

I am trying to analyze a single cell sequence using GSE148434 data.

I want to analyze using the 'Seurat' package, but I don't know how to do it.

I succeeded in loading the 'h5' format file. Here is my code.

untar("GSE148434_h5_RAW.tar")
h5.fileList <- list.files(pattern = "info.h5")
load_h5_file <- function(h5.fileList) {
  h5_data <- h5read(h5.fileList, "/")
  return(h5_data)
}
all_h5_data <- lapply(h5.fileList, load_h5_file)

And this is one of the data examples included in 'all_h5_data'. 'all_h5_data' is a 'Large list' format in R.

I don't know how to perform single cell rna sequencing analysis using the 'Seurat' package.

Please help me.

[Hamid Ghaedi]

I would recommend first reading background material on the topic and then replicating the tutorials available from Seurat developers; For start, you may check this out: https://satijalab.org/seurat/articles/pbmc3k_tutorial.html. You may ask your question if any, while replicating the tutorial here.