How to pass from DNA to AA fasta

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18 months ago

Anderson Stiward • 0

Hi,

I hope you are well. I am trying to figure out how can I pass a eukaryotic gene that has introns to aa sequence. The only way that I have thought is to transcribe all the gene then translate it with the ORF that is correct, and then do a blast for proteins. With the highest identity% entry make an alignment and then cut the parts that do not have in common.

Any other idea, please?

Thank you for your help and time.

genetics • 1.2k views

ADD COMMENT • link updated 18 months ago by Buffo ★ 2.4k • written 18 months ago by Anderson Stiward • 0

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If you know the intron sequence, remove it and use ORFfinder

ADD REPLY • link 18 months ago by Buffo ★ 2.4k

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Do you know another alternative If I do not have the sequence of the intron?

ADD REPLY • link 18 months ago by Anderson Stiward • 0

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If it is only one gene, maybe you can try to find it manually using the GT/AG universal intron motifs.

ADD REPLY • link 18 months ago by Buffo ★ 2.4k

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What do you mean by "pass" in the context of a gene?

ADD REPLY • link 18 months ago by Ram 44k

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Hi i mean to convert it from DNA to AA, to do an in silico translation

ADD REPLY • link 18 months ago by Anderson Stiward • 0

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You should have just said "translate to AA" then, "pass" doesn't really mean anything.

ADD REPLY • link 18 months ago by Ram 44k

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