Entering edit mode
19 months ago
ALOUSH ALI
•
0
I am using DEGseq package with DEGexp with MARS option. So i am trying to recall the example that the manual provide
library(DEGseq)
geneExpFile <- system.file("extdata", "GeneExpExample5000.txt", package="DEGseq")
geneExpMatrix1 <- readGeneExp(file=geneExpFile, geneCol=1, valCol=c(7,9,12,15,18))
geneExpMatrix2 <- readGeneExp(file=geneExpFile, geneCol=1, valCol=c(8,10,11,13,16))
head(geneExpMatrix1)
Results
## EnsemblGeneID R1L1Kidney R1L3Kidney R1L7Kidney
## ENSG00000146556 "ENSG00000146556" "0" "0" "0"
## ENSG00000197194 "ENSG00000197194" "0" "0" "0"
## ENSG00000197490 "ENSG00000197490" "0" "0" "0"
## ENSG00000205292 "ENSG00000205292" "0" "0" "0"
## ENSG00000177693 "ENSG00000177693" "0" "0" "0"
## ENSG00000209338 "ENSG00000209338" "0" "0" "0"
## R2L2Kidney R2L6Kidney
## ENSG00000146556 "0" "0"
## ENSG00000197194 "0" "0"
## ENSG00000197490 "0" "0"
## ENSG00000205292 "0" "0"
## ENSG00000177693 "0" "0"
## ENSG00000209338 "0" "0"
layout(matrix(c(1,2,3,4,5,6), 3, 2, byrow=TRUE))
par(mar=c(2, 2, 2, 2))
DEGexp(geneExpMatrix1=geneExpMatrix1, geneCol1=1, expCol1=c(2,3,4,5,6), groupLabel1="kidney", geneExpMatrix2=geneExpMatrix2, geneCol2=1, expCol2=c(2,3,4,5,6), groupLabel2="liver", method="MARS" , output = "DEGseq")
## Please wait...
## gene id column in geneExpMatrix1 for sample1: 1
## expression value column(s) in geneExpMatrix1: 2 3 4 5 6
## total number of reads uniquely mapped to genome obtained from sample1: 345504 354981 334557 366041 372895
## gene id column in geneExpMatrix2 for sample2: 1
## expression value column(s) in geneExpMatrix2: 2 3 4 5 6
## total number of reads uniquely mapped to genome obtained from sample2: 274430 274486 264999 255041 284205
##
## method to identify differentially expressed genes: MARS
## pValue threshold: 0.001
## output directory: none
##
## The outputDir is not specified!
## Please wait ...
## Identifying differentially expressed genes ...
## Please wait patiently ...
## output ...
## The results can be observed in directory: DEGseq
So when I open the output I only can see the value of genes in the two groups ("kidney and " "liver")
My question is there any way to view the value of the gene counts in each sample like kidney1, kidney2, kidney3
Your post is badly formatted, you should enhance the presentation. I do not understand your question because you created geneExpMatrix1 and geneExpMatrix2 which corresponds to the read counts and you are asking where you can view it
My question is about the output(differential gene expression analysis results) which contains results of logfoldchange and pvalues between the two groups (kidney and liver). So my question if there is a way to generate an output show those values between the samples among the groups like R1L1Kidney, R1L3Kidney, R1L7Kidney and liver1, liver2, liver3
logFC and p-values are results of a differential analysis between the groups, it does not exist in the context of individual samples