I have one fasta file of a bacteria. Can i use to create a phylogenetic tree?

Question

[Mustafa]

I am new to bioinformatics. I have identified an organism of klebsiella. So i was wondering if i can make a tree from it?

Answer 1

[andres.firrincieli]

If the fasta file contains the genome sequence you can use the following:

1. GToTree (see example here)
2. GTDB-tk de novo workflow
3. TYGS. Just upload the genome and wait until the analysis finish

GOToTree and GTDB-tk are not user-friendly but allow you to build a phylogenetic tree using all the type-strain (representative strains) of your genus.

The TYGS server instead is much easier to use but the phylogenetic tree is built using the top 10 type-strains that are more closely related to your strain

[Mustafa]

Thank you so much ! will try them all !