Entering edit mode
17 months ago
ju_ra
•
0
Hi guys,
I am trying to map online scRNASeq Data to the murine genome using STAR (Galaxy Version). Looking at my results I get the following output:
Barcodes:
noNoAdapter 0
noNoUMI 0
noNoCB 0
noNinCB 0
noNinUMI 0
noUMIhomopolymer 0
noNoWLmatch 0
noTooManyMM 0
noTooManyWLmatches 0
yesWLmatchExact 0
yesOneWLmatchWithMM 0
yesMultWLmatchWithMM 0
Genes:
noUnmapped 34353576
noNoFeature 49132949
MultiFeature 35781538
subMultiFeatureMultiGenomic 31881499
noTooManyWLmatches 0
noMMtoWLwithoutExact 0
yesWLmatch 201362414
yessubWLmatchExact 201362414
yessubWLmatch_UniqueFeature 201362414
yesCellBarcodes 1561374
yesUMIs 125622644
Does anybody have an Idea why I get so many cells? As I understand it correctly yesCellBarcodes means the number of cells which were detected but 1561374 seems way to high...