Does Minknow in nanopore sequencing trim low qscore bases?
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17 months ago
grkorompis • 0

Does Minknow in nanopore sequencing trim low q-score bases while running the sequencing when the q-score threshold is set? Because if that's the case, I was thinking that it would split the read as well, if for some reason the low quality base is found in the middle of the reads.

Alternatively, I assumed that instead of trimming low quality bases, it would instead average q sqore per read, and for any of those read who has low average q score than the threshold set in the minknow, then those reads would be fitered out.

Thank you.

filtering nanopore qc minknow ont • 494 views
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