How to convert Kallisto output to Salmon?
1
0
Entering edit mode
17 months ago

What's the best way to do this? I am looking to produce the salmon .sf file.

quantification rnaseq pseudo-alignment • 786 views
ADD COMMENT
0
Entering edit mode
ADD REPLY
0
Entering edit mode

thanks, maybe i'm going crazy but i don't see an exact answer to the question i asked in those search results. the first result shows how to convert salmon to kallisto (the opposite) so it can be used in sleuth. the rest are how to import either kallisto or salmon into R for edgeR or tximport. As far as i can see these methods do not take the .h5 file and output the .sf file. maybe my mistake is in assuming there exists such a method that i don't have to make myself.

ADD REPLY
2
Entering edit mode
17 months ago
dsull ★ 6.9k

kallisto's abundance.tsv and salmon have basically the exact same columns (transcript, length, effective length, TPM, counts, etc.) -- why not just rename & reorder the columns?

ADD COMMENT
0
Entering edit mode

makes sense, i didn't realize kallisto had a plaintext option. printed a plaintext option by default

ADD REPLY

Login before adding your answer.

Traffic: 1877 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6