vector::reserve error message (Problem solved)

Question

[Andy]

Hello,

I need help to solve this error message. It really concerned me.

At first, I meet the error message:

vector::reserve.

When I tried to merge multiple Seurat objects.

Now I am running fastMNN function, still meet exact same problem. I am wondering probably where is the problem. I am sure all Seurat objects are correctly established. To trouble shot this problem, what is your guys suggestions?

data_list<- RunFastMNN(object.list = data_list)
Computing 2000 integration features
Error: vector::reserve

Thanks a lot!

Andy

[Andy]

It is possible because of the file size? I successfully used Python to merge my data, which totaled more than 10 GB, without performing any integration analysis.

[Andy]

UPDATE: Yes, my problem is likely caused by my dataset size being larger than what Seurat can handle. Stuart, on the other hand, can handle a size of a sparse matrix in R, up to 2^32-1 non-zero entries.