Presence of ribosomal gene / mitochondrial gene enrichment in each cluster of scRNA seq analysis

0

Entering edit mode

16 months ago

applepie ▴ 10

Hello Everyone, recently I have been working on the scRNA seq analysis of HBV-associated Heptatocellular carcinoma (HCC) cells using Seurat packages. When I used the 20 genes with lowest adjusted p-value in each cluster to plot the violin plots and Feature Plots, I realized that some mitochondrial genes and ribosomal genes are highly enriched in every cluster (as shown in the attached photo). Regarding this, I have several questions:

I think It should make sense and be inevitable to see the mitochondrial gene & ribosomal gene enrichment, even in the presence of quality control to filter cells with high % of mitochondrial genes. You know, mitochondrial and ribosomal genes should be present in all the kinds of cells because they serve essential functions.

scRNAseq • 769 views

ADD COMMENT • link updated 16 months ago by Nitin Narwade ★ 1.6k • written 16 months ago by applepie ▴ 10

0

Entering edit mode

enter image description here

ADD REPLY • link 16 months ago by applepie ▴ 10

1

Entering edit mode

What is your question?

ADD REPLY • link 16 months ago by Nitin Narwade ★ 1.6k

Login before adding your answer.