Which KEGG Database to use for DIAMOND annotation of predicted gene coding regions?

Question

[Lily]

Hello,

I am new to bioinformatics and working on a metatranscriptomics pipeline and would like help with understanding how to do functional annotations. I currently have predicted gene coding regions using Prodigal and would now like to use DIAMOND in order to obtain functional annotations against the KEGG database. I have access to the new KEGG database but was unsure of the all the differences between the multiple databases provided by them.

Would someone be able to tell me which database is the appropriate choice to use for this? I would assume it is the KEGG gene database but after reading the ReadMe File about the gene database folder, I was left unsure about the specific contents in it.

Thank you.

Answer 1

[Asaf]

Although it's a good exercise, I would recommend using existing tools like prokka