Entering edit mode
16 months ago
ashkan
▴
160
I have count matrix scRNAseq data from cel-seq2 (96 well plates)
protocol for few samples which are tab separated text files in which the rows are genes and the columns are cells from different wells in the plate.
I have no experience of working with cel-seq2 which is different from 10X genomics. I found a tool with the same name from here:
https://github.com/yanailab/celseq2
but so far I did not manage to get it working. since I already have count matrix, is it possible to use Seurat R
package for this data? if so, how can I load this data to a Seurat object
You can use
CreateSeuratObject
: https://www.rdocumentation.org/packages/Seurat/versions/3.0.1/topics/CreateSeuratObject