Reference genome assembly

Question

[singhankit973]

This is the data i have and i have retrieved the sequences and mapped the reads with respect to reference genome. Now what should I do after mapping reads???

1. ICC9755
2. ICC19147
3. ICC12299

4. ICC4639

   • Reference genome guided assembly
   • Please use the ref. genome sequence of desi type Chickpea (Cicer arietinum) which is available in NCBI genome database as Cicer arietinum ASM33114v1 and Submitter: BGI-Shenzhen.
   • NCBI link for the Ref. Genome Seq is https://www.ncbi.nlm.nih.gov/genome/?term=Cicer+arietinum .
   • GFF file of reference genome is available in the above given link

[Ram]

I'm deleting this post as it's a duplicate of How can I approach to this problem, pls help. Reference genome assembly.

EDIT: I've reopened it as OP mentions that this is a different question: How can I approach to this problem, pls help. Reference genome assembly.

[dthorbur]

This sounds a little bit like a bioinformatics assignment to me.

Typically, unless you've shown an attempt to understand and solve the issue you won't get productive answers on here. Also I don't see much difference between the two posts, but Ram did reopen, so maybe I've missed something.

My broad suggestion is to look up recent published studies that have achieved what you are looking to do to find a general workflow and suitable tools. ref and ref, for example.