Hi everyone,
I have RNAseq data from epithelial cells and I'm currently working on understanding their cell state using a >20 genes signature for differentiating between Basal, club cells, mucus-producing goblet cells, and ciliated cells. I want to convert the count of transcripts into a cell differentiating score, similar to what is done for mitochondrial genes and calculating % in Seurat.
Q1: I'm considering taking the sum of transcripts from the 20 genes signature and calculating the % to obtain a score. Does this approach seem appropriate and reliable?
Q2: Additionally, I'm wondering if there is already a method or existing tool that can help assign the expression of a set of genes to specific cell types, allowing for the differentiation between different cell types based on the expression of distinct gene sets?
Looking forward to your insights and suggestions. Thank you!
