Hi there,
I am writing to ask if there is a way to convert the SeuratV4 object to the SeuratV5 object.
My colleague and I are working together trying to integrate existing public scRNA-seq datasets to create a scRNA-seq atlas for a specific disease. My colleague already downloaded all the datasets we need and generated SeuratV4 objects for each study. My job now is to integrate all the Seurat objects and do downstream analysis. Since the integrated dataset will be very big, I am trying to use SeuratV5 rather than SeuratV4 to do the integration.
I read the SeuratV5 vignettes from the development team and found that if I want to create a SeuratV5 object, I have to rerun the Cell Ranger and so on. I wonder if this means that I need to redo all the work that my colleague already did (starting from the 10x results downloaded online)? Or is there a good way to direct convert the SeuratV4 object to the SeuratV5 object?
Thank you so much for your reading! I am looking forward to your reply.
Best wishes Kexin
Have you had a look at the function
UpdateSeuratObject?More info here