Retrieve deseq2 normalised counts with only results file available
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16 months ago
BM ▴ 70

I have several Deseq2 results file, so a csv file with ensembl name, baseMean , log2FoldChange, lfcSE, stat, pvalue, padj columns. Unfortunately, I don't have the original count files.

Is there a way to extract Deseq2 Normalised Counts from the results file only? Is this possible?

Thanks in advance

RNAseq Deseq2 Normalised Counts • 692 views
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Entering edit mode
16 months ago
ATpoint 85k

No, this information is lost.

Note that without counts and raw data at hand your analysis is not reproducible, so treat results with care. I would personally not even use the results productively other than for internal hypothesis generation since you can never rerun analysis.

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16 months ago
Ayeh • 0

unfortunately, No, its not possible. if you have fastq files or every intermediate files before construction of count matrix, you can do all thing at first to great your count matrix again.

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