Multi copy gene

Question

[p20180001]

Hi,

I am looking for guidance. I have been assigned a task to find the relative copy number of ribosomal RNA genes in humans using qPCR.

I decided to choose a couple of single-copy genes as references to start with. However, I also have a parallel thought that I might as well consider specific multiple-copy genes. But as I search, I am unsure how to select multiple copy genes as no research gives the slightest idea of these gene copy numbers, or I'm unaware of a database that can give a rough figure of the actual copy numbers, for example, tRNA or alpha-tubulin gene there is multiple research that mentions they are multiple copies but no ballpark figure.

Could anyone please suggest or let me know if I am heading in the right direction?

[German.M.Demidov]

https://www.nature.com/articles/ng.3200 not exactly what you look for, but gives a good insight

however many paralogous genes are paralogous already in the reference

recent Human PanGenome Consortium work addresses this https://www.nature.com/articles/s41586-023-05896-x - Fig. 2

[GenoMax]

I have been assigned a task to find the relative copy number of ribosomal RNA genes in humans using qPCR.

rDNA repeats are a tricky question to address. There may be tens (or hundreds) of copies.

https://www.nature.com/articles/s41598-021-82565-x

Additional references: how can i download human ribosomal reference ?