how to solve the problem "Invalid character (+) in sequence"

Question

[alice005201314]

When I annotated the metagenomic data with args_oap_3.2.3, the following error was reported.

I found the contents of the line in the corresponding file as follows. I don't understand what the problem is, how to solve it.

[MatthewP]

Your fastq file is broken, the line after @SRR10492803.32660369 32ACATTTT... is +, this line should be sequences line, not +. You need to use md5sum to check md5 files after downloading data.

[alice005201314]

Thank you. I thought that might be the reason

[5heikki]

The program expects a fasta file but you're giving it a fastq file?

[alice005201314]

The fastq files are also available