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16 months ago
scRNA2023
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Hi Members, I need to do an assignment in single cell RNA seq using the tools tagged above. I will appreciate if anyone can help me out. I have done basic scRNA analysis but need guidance on some of the tools.
Thank you in advance
Please understand that these open-ended Qs are difficult to answer. Read the manuals and help pages and then edit the Q with a specific problem.
Hi ATpoint..
Thank you for the suggestion. I am familier with basic scRNAseq pipeline using Seurat. However, I am required to reanalyzed a published scRNA Seq data using the following tools: Doubletfinder, Harmony, Cell Typist, CellO, scGATE, Monocle, SoupX, Cellbender, scTransform
Hence, I wish to have a tutor who can help me out with this. I can be contacted at scrna2023@gmail.com Thank you
Do not add answers unless you're answering the top level post. Use
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instead as appropriate. I've moved your post to the right location this time.Thank you for this Ram. Highly appreciated.
Try something, then ask specific questions. It's not a code writing service here, nor do people actively contact you via email. That's just not how communities like this work.
Hi ATpoint,
Yes I understand and I am trying to go through the documentations of respective packages also. However, I was just exploring if anyone might be interested to teach :-).
Are you interesting in paying this someone that might be interested in teaching you?
Yes of course..
There are free as well as paid courses online, why ask for this on a question you opened? Why not create a job posting with all relevant details? That will attract the users that freelance.
yes.. you are right. I have seen similar posts here in Biostars also and though that i might find anyone willing to freelance..
Those of us who reach the peak of our game invest much time in self-learning... driving ourselves each day to do better... to learn more.
There are many scRNA-seq tutorials available online for these tagged 'tools'. May you please conduct a search and then report back [here] with a bullet-point list of these tutorials - this would greatly assist the community.
Hi, Yes sure, I can do a search and list down the tutorials for these tagged tools. But mostly, I am going through the documentations of these tools only.
Thank you. May you have the report for me by Monday midday your local time? I would appreciate it. Thank you.
What is the difference between the filtered and raw gene-barcode matrix?
This is well-earned: https://letmegooglethat.com/?q=10x+cellranger+filtered+vs+raw