Dear all,
we have the last 4 seats available for the course "Introduction to CRISPR for ecology and evolution studies": https://www.physalia-courses.org/courses-workshops/course53/
Dates: online, October 9th-13th
This course aims to equip participants with the knowledge and skills necessary to effectively utilize the CRISPR-Cas9 system for evolutionary studies. . This course will include both fundamental theoretical lessons and hands-on sessions with practical examples of genomic data analysis. All data used in this course has been chosen from up-to-date scientific literature. It will focus on experiment design, selection of methods and data analysis. General “starting-point” bench protocols will be shared, and each day will include a “Discussion and Q&A” session where participants can fine-tune their own projects with the instructors.
LEARNING OUTCOMES
• Understand the fundamentals of the CRISPR-Cas9 genome-editing tool.
• Design a reliable CRISPR-based gene knockout experiment.
• Annotate a gene of interest in a non-model species.
• Design and evaluate species-specific single guide RNAs (sgRNAs).
• Efficiently synthesize sgRNA and assemble optimized Cas9/sgRNA mixes.
• Screen for G0 CRISPR mutants using a fast genotyping method.
• Design genotyping strategies according to project needs.
• Analyze Cas9-induced genomic variants by SANGER and Illumina deep sequencing.
For the full list of our courses and workshops, please have a look at: https://www.physalia-courses.org/courses-workshops/
