RNA-Seq

Question

[prs]

I have a strand specif RNA-Seq data with strand-specific RNA-Seq bam files. Now, I want to map positive and negative gene locations in gtf file, on bam file. So, should I map positive locations on reverse bam and vice versa?

[seidel]

What are you actually trying to achieve with your data? Why do you have already mapped data that you now want to map a second time? Have you searched the forum (i.e. this discussion might be helpful?)?