Entering edit mode
16 months ago
Chris
▴
340
Hi all, I decrease the resolution to get fewer clusters however I got the error when run:
FeaturePlot(object_combined, features = c('PECAM1'), min.cutoff = 'q10')
Error: None of the requested features were found: PECAM1 in slot data
Would you please have a suggestion? I don't have the error when keep high resolution. I appreciate it.
I think it is:
I would recommend you to check in each data slots.
When I run
DefaultAssay(object_combined) <- "RNA"
, the error has gone. Thank you!when I choose
SCT
asDefaultAssay
instead ofRNA
. theFeaturePlot
is very different. Would you please have a comment? I found this one but still not clear: Seurat3: RNA vs SCT assays for DotPlot