How to remove chimera from 16S Sanger contigs
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14 months ago

Hi,

I have a bunch of 16S Sanger sequences, and I used UGENE to trim and edit sequences, then generated the contig using CAP3 embedded in UGENE. I submitted most of the sequences in the GenBank and received their accessions, but a few of them failed to be submitted and I received a uchime report for the chimeric sequences detected in these contigs. Could you please help me know how to use this report to clean the contigs from chimeric sequences?

Thanks!

sanger-sequencing chimera uchime • 353 views
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