Software Tool to Translate DNA to Protein

Question

[sil_bioinfo]

Hello,

I'm looking for any software tool to translate DNA sequences (big fasta files) to Protein with all 6 frames in the Standard Code. I tried the Expasy translate tool, EMBOSS Transeq,... but my files are too big (68.1 MG). I'm using a mac!

Thank you in advance

Answer 1

[biofalconch]

You could give seqkit a go, can be run using Windows. Something like this should work:

seqkit translate <YOURFILE> --frame 6 --append-frame 

For more info read the documentation here:https://bioinf.shenwei.me/seqkit/usage/#translate

[sil_bioinfo]

It worked, thank you so much for your help!

Answer 2

[GenoMax]

but my files are too big (68.1 MG)

You can install EMBOSS locally (unix machine required) and run transeq that way.

[sil_bioinfo]

Hello, it works on mac too?

[GenoMax]

It should: https://ports.macports.org/port/EMBOSS/

https://abittechnical.work/en/steps-to-install-embl-ebi-emboss-on-apple-silicon-mac/

[sil_bioinfo]

It worked too, thank you so much for your help!