ChIP seq BamCoverage

0

Entering edit mode

14 months ago

lonekaisar72 • 0

Dear All,

We are trying to analyse some ChIP seq data sets on one of the repeated regions of Genome (Ribosomal DNA). Recently one paper defined the pipeline for this region, upon following the pipeline we are not getting the tracks as expected for the data set already used in the paper. Upon following the whole pipeline we are getting same tracks for our input and IP.We suspect that we are getting wrong in the BamCoverage command. For further convenience, we have attached the link of the manuscript which we are following. Also we have attached the screen shot of tracks.

enter image description here

Link for the paper https://doi.org/10.1016/j.jbc.2023.104766

ChIP-seq • 601 views

ADD COMMENT • link updated 14 months ago by Ram 44k • written 14 months ago by lonekaisar72 • 0

0

Entering edit mode

You have to scale the tracks to same height. Select all tracks, then right-click and select auto-scale. Read the IGV manual.