KEGG Pathway analysis for non-model organism using goseq
0
0
Entering edit mode
13 months ago
analyst ▴ 50

Dear all,

I have to perform KEGG Pathway analysis of wheat DEGs (differentially expressed genes). I found goseq in R but wheat is not native organism. For GO term analysis of wheat I first retrieved GO term IDs from biomart and then fed to goseq function but I did not find KEGG in biomart. How can I get KEGG ontologies?

Anyone please suggest how can I perform KEGG Pathway analysis for non-native organism's genes.

Thanks

non-model-organism KEGG DEG • 1.0k views
ADD COMMENT
1
Entering edit mode

You need to use KEGGREST to retrieve the KO for wheat. See this question for more details: KEGG pathways associated to a gene using BiomaRt

ADD REPLY
0
Entering edit mode

Thanks Mark I tried this thread. For short list of genes it worked well but gave timeout error for larger list (containing thousands of genes). I used enrichKEGG it worked for larger list.

ADD REPLY

Login before adding your answer.

Traffic: 1440 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6