RNAseq with panel of normals
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14 months ago
Jay • 0

A naive n00b question here. Title says it. We have some tumor samples that we're planning to sequence with a Nanostring 360 panel. We have paired normal for each sample but we also have a lot of samples. Could we get away with running fewer normals and creating a panel of normals or is running paired tumor/normal heavily advised? I've been poking around and can't find anything on this. Thanks!

rna-seq panel-of-normals • 531 views
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What is the analysis goal?

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Just to discover differentially expressed genes between sets of cancer samples based on methylation status, histology type, etc. We're just looking for genes that stand out between the two groups.

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