Entering edit mode
14 months ago
Nico80
▴
80
I am starting to work with some single cell sequencing data and I am trying to get my head around what the best pipeline is.
I have 6 samples in total, 3 from control and 3 from a treated group. Of each, 2 were done with a 10x multiomics kit (scRNAseq and scATACseq) and the other is only scRNAseq.
I was going to align the multiomics samples using cellranger-ARC and the other sample using 'vanilla' cellranger, then process everything in Seurat.
However I'm not sure if
- that's the best way of proceeding
- how do I go about putting everything together (and whether it is possible). I'm going to align samples separately and then use
cellranger aggr
orcellranger-arc aggr
. Would I then be able to import everything onto a single Seurat object or what's the best strategy?