error in Fastq

Question

[lonekaisar72]

HI I am trying to do fastqc of one file but after running approximately 100 percent it is showing the below-listed error Please suggest what can be done. Thanks

Approx 100% complete for SRR18330177_2.fastq
It seems our guess for the total number of records wasn't very good.  Sorry about that.
Still going at 105% complete for SRR18330177_2.fastq
Still going at 110% complete for SRR18330177_2.fastq
Still going at 115% complete for SRR18330177_2.fastq
Failed to process file SRR18330177_2.fastq
uk.ac.babraham.FastQC.Sequence.SequenceFormatException: Midline 'F:GAATAA7FFFFFFFFFFFFCCCTCGCCCACCATTCCCAAACTCFFFFF:F,F:F,,' didn't start with '+' at 72176579
        at uk.ac.babraham.FastQC.Sequence.FastQFile.readNext(FastQFile.java:179)
        at uk.ac.babraham.FastQC.Sequence.FastQFile.next(FastQFile.java:129)
        at uk.ac.babraham.FastQC.Analysis.AnalysisRunner.run(AnalysisRunner.java:77)
        at java.base/java.lang.Thread.run(Thread.java:829)

[Pierre Lindenbaum]

"everyone" is not a tag. Change it please.

[Pierre Lindenbaum]

what are the outputs of

file SRR18330177_2.fastq

and

head SRR18330177_2.fastq

[bk11]

Your file is corrupted. Better to get fresh copy of it.

Answer 1

[GenoMax]

Midline 'F:GAATAA7FFFFFFFFFFFFCCCTCGCCCACCATTCCCAAACTCFFFFF:F,F:F,,' didn't start with '+' at 72176579

Looks like your file is corrupt with at least one malformed fastq record. Please redownload.

[lonekaisar72]

Thanks sorted

[Ram]

Fix this post by adding a proper tag and removing the everyone tag.