Entering edit mode
12 months ago
muhammad.akram
▴
10
Dear, I i am using q2ONT workflow for the Nanopore seq data. I am also using it for my 16srRNA gene seq data. I am facing a problem with dereplication of the sequences. When i see my 5-derep-table.qzv, i get all the reads as unique features and they are equivalent to number of reads in my samples. This way, frequency per features becomes 1. Attached figures for your reference. I did demultiplxing and adapter trimming with Porechop and filtering with trimmomatic. Could you please suggest where it it went wrong?
