Bowtie2 Error: reads file does not look like a FASTQ file

Question

[SO_Bio]

Hi, I need your help. I am using this code and bowtie sends me this error. Thank you

bowtie2 -x /home/anam/HumanGenome/HomoGenbt2/HumanGenome -1 14iadR1_forward_paired.fastq -2 14iadR2_reverse_paired.fastq --un-conc-gz 14iadR_host_removed -S 14iadR_mapped_and_unmapped.sam

Error: reads file does not look like a FASTQ file
terminate called after throwing an instance of 'int'
Aborted (core dumped)
(ERR): bowtie2-align exited with value 134

[ATpoint]

head 14iadR1_forward_paired.fastq

?

Side note, gzip your files, uncompressed files just take space but that's minor.

[Josefina]

Hi! I used my files as gzip and I am having the same error. Could you fix it?

[ATpoint]

Use file and head on a fastq file that errors, make sure it is really gzipped and not correpted.