How to obtain full alignment results?
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13 months ago
nrqstudent • 0

Hi, When I prepare to align my short reads to the human pan-genome graph genome, the result is only a path, 1. what I want is to provide me all the sequences that have a mismatch and full alignment with this reads?

  1. And how can I know the annotation information of the sequences that are aligned to the reads, such as to exon regions, cds regions or intron regions, from the results?

Shell:
vg giraffe -Z hprc-v1.1-mc-grch38.gbz -p -f ./small_sim.fq -o json--max-multimaps 10 >small_sim_aln_M10.json
Results:
enter image description here

giraffe vg • 340 views
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