KEGG module and abundance of its KEGG Orthologs for functioning

Question

[Jonathan Yoou]

Hi all,

I'm trying to interpret KEGG Orholog (KO) abundance data from two different groups.

As you know, KEGG module is composed of multiple KOs. Then, if I want to say "this KEGG module functions well and actively", does it always have to have all high abundance of KOs comprising it?

I mean, in case of transcription of DNA, for example, it needs one helicase while multiple polymerase can be used for transcription, which means low helicase, super high polymerase can be interpretted as "transcription is active!".

Like that, how can I make interpretation considering it (maybe efficiency?)? Is there any database or tool I can draw conclusion given them? Or wet experiment for check them is the only way?