Entering edit mode
13 months ago
Aki
▴
20
Hi. I am analyzing TCGA methylation data from TCGAbiolinks and I faced one problem during annotation process with annotatr. This TCGA data has covered a gene in the chromosome 19, but annotated result did not contain one region in chromosome 19. I used the opensea region probe for compartment analysis and there is no opensea probe for these genes in missing region. The genes are covered by only Island, N_Shore, and S_Shore region probe. Is that because, I couldn't observe the annotation result of the gene? I used following script. Thank you for your help.
library(minfi)
compartments_result <- compartments(GRset, chr = paste0("chr", 19), what = "OpenSea", resolution = 100 * 1000)
library(annotatr)
annots = 'hg19_basicgenes'
annotations = build_annotations(genome = 'hg19', annotations = annots)
AB_annotated_results <- annotate_regions(regions = compartments_result,
annotations = annotations,
ignore.strand = TRUE,
quiet = FALSE)