How to determine E. coli MLST (Multi Locus Sequence Typing ) from the sequencing of the PCR product of 7 housekeeping genes

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12 months ago

Ralph • 0

Hi,

I am working on a project to determine antibiotic resistance origins in E. coli. We have amplified 7 housekeeping genes using PCR and sequenced the product on Nanopore MINIon. I would like to determine the MLST profile for each isolate. I have 1 run for a barcoded 96 well plate with each well with one isolate. basecalling and demultiplexing are already done. I am not being able to move further.

Thank you for the help

MLST phylogenetic nanopre • 1.1k views

ADD COMMENT • link updated 12 months ago by Karen • 0 • written 12 months ago by Ralph • 0

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Give the sequences to an MLST server or commandline tool on a per-sample/isolate basis.

The tool will specify the format it wants - probably a multifasta

ADD REPLY • link 12 months ago by Joe 21k

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To complement Joe's answer: here is a webserver: https://cge.food.dtu.dk/services/MLST/ and a commandline tool: https://github.com/tseemann/mlst

ADD REPLY • link 12 months ago by Karen • 0

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