DoubletDecon - improved_Seurat_Preprocessed() gives error
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Entering edit mode
11 months ago
kat.bi • 0

I've a SeuratObject Leaf_frozen and I'm using this data into DoubletDecon's Improved_Seurat_Pre_Process() as shown below-

Leaf_processed <- Improved_Seurat_Pre_Process(Leaf_frozen, num_genes=50, write_files=FALSE)

which throws output and error -

Validating object structure
Updating object slots
Ensuring keys are in the proper structure
Ensuring keys are in the proper structure
Ensuring feature names don't have underscores or pipes
Updating slots in RNA
Validating object structure for Assay5 ‘RNA’
Object representation is consistent with the most current Seurat version

*Error in as.data.frame(seuratObject@assays[["RNA"]]@CountS) :
no slot of name "counts" for this object of class "Assay5"*

This link shows detailed information on the said issue raised before if needed - **satijalab/seurat#8210**

Please suggest a solution.

doublet-detection sc-genomics single-cell • 842 views
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Entering edit mode

Is there a reason you did not mention that you also opened this issue on GitHub? https://github.com/EDePasquale/DoubletDecon/issues/56 ?

Also, this is rude:

Please suggest a solution soon.

Your urgency is your problem, not anyone else's.

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