Entering edit mode
11 months ago
Sanjukta
•
0
This post is a continuation of my previous discussion here. I tried Galaxy to analyse miRNA-seq data and not miRdeep2 as was suggested, due to some temporary issues of internet at my campus.
My miRNA fastq files read as follows:
>SRR1658346.1 HISEQ1:187:D0NWFACXX:3:1101:2565:2050 length=51
ATCATACAAGGACAATTTCTTTTAACGTCGTATGCCGTCTTCTGCTTGNAA
>SRR1658346.2 HISEQ1:187:D0NWFACXX:3:1101:2654:2232 length=51
TCGAGGAGCTCACAGTCTAGTATAACGTCGTATGCCGTCTTCTGCTTGAAA
>SRR1658346.3 HISEQ1:187:D0NWFACXX:3:1101:2870:2103 length=51
TTCAAGTAATCCAGGATAGGCTAACGTCGTATGCCGTCTTCTGCTTGAAAA
>SRR1658346.4 HISEQ1:187:D0NWFACXX:3:1101:3001:2147 length=51
TAGCACCATCCGAAATCAGTTTAACGTCGTATGCCGGCTTCTGCTTGAAAA
The second sequence is probably my adaptor sequence:
ATCATACAAGGACAATTTCTTT TAACGTCGTATGCCGTCTTCTGCTTGNAA
TCGAGGAGCTCACAGTCTAGTA TAACGTCGTATGCCGTCTTCTGCTTGAAA
TTCAAGTAATCCAGGATAGGC TAACGTCGTATGCCGTCTTCTGCTTGAAAA
TAGCACCATCCGAAATCAGTT TAACGTCGTATGCCGGCTTCTGCTTGAAAA
But on doing FastQC and MultiQC, "No samples found with any adapter contamination > 0.1%" this message is displayed.
The individual FASTQC file however predicted the occurrence of Adaptor:
Is the alignment of the sequence wrong? Is it supposed to start with the adaptor sequence? So should I rewrite the fastq files?
Galaxy specific questions are best asked on their help forum: https://help.galaxyproject.org/
Here are some past threads there that may be of help: https://help.galaxyproject.org/tag/mirna