need some recommendation for missense mutation insilico analysis

Question

[sindy_roby]

Hello everyone

I need some help to do my in silico analyses. I want to analyze some missense mutations. I have already investigated the effect of mutation on protein stability. I have also checked the rate of pathogenesis of mutations by using pathogenesis prediction tools. Now I want to know whether my mutations affect the process of phosphorylation or glycosylation or not. Does the creation of a new cysteine amino acid have an effect on other disulfide bonds and if so, what is the effect? I want to know if these mutations change protein folding or not. it should be noted that my mutations do not change the second structure of the protein.

I would be grateful if you could guide me

Thank you in advance for your answers

Answer 1

[Mensur Dlakic]

There is no general answer to your question, as mutations may affect only one aspect of the structure-function relationship. Mutations of catalytic residues will usually not affect the structure, but may completely kill the function. Likewise, a mutation away from the active site (e.g., sickle-cell disease) may considerably perturb the structure without completely killing the function.

These questions can only be answered by understanding functions of individual proteins. Don't know what you have tried already, but Rosetta allows in silico modeling of proteins.

https://www.rosettacommons.org/software