Metagenomics mock datasets

Question

[enuhblaise]

Hello all,

Anyone has a procedure for creating mock metagenomic datasets, or any resources where I can find already made datasets.

Answer 1

[Mensur Dlakic]

A general way of making a mock metagenome is to collect all individual genomes of interest, have them represented in a certain ratio to simulate abundance, and create simulated sequencing reads. Googling should give you some ideas how to do it.

If you are interested in existing datasets, I suggest you read these two papers and consult their resources:

• https://www.nature.com/articles/nmeth.4458
• https://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-019-0633-6
• https://github.com/CAMI-challenge/CAMISIM
• https://data.cami-challenge.org/

[enuhblaise]

Thank you @Mensur Dlakic ,

This is very helpful