Entering edit mode
11 months ago
Assa Yeroslaviz
★
1.9k
I was wondering where I can find explanation about the output given to me by homer's makeTagDirectory
command.
one of the files return this output:
Optimizing tag files...
Estimated genome size = 4227865
Estimated average read density = 1.741583 per bp
Total Tags = 7363177.0
Total Positions = 11846383
Average tag length = 175.6
Median tags per position = 0 (ideal: 4)
Average tags per position = 0.197 <=?
Fragment Length Estimate: 191
Peak Width Estimate: 0 <=?
!!! No reliable estimate for peak size
Setting Peak width estimate to be equal to fragment length estimate
Autocorrelation quality control metrics:
Same strand fold enrichment: 1.0
Diff strand fold enrichment: 1.0
Same / Diff fold enrichment: 1.0
Guessing sample is ChIP-Seq - may have low enrichment with lots of background
Forcing fragment length = -123456
How do I interpret these results? Is it correct to assume here, that the ChIP-seq experiment wasn't really successful? or why do i get this message at the ensd?