BLASTx Number of Matches

Question

[suyuqi]

Hi all, can anyone help me out to understand why this happens in blastx?

I get more than one matches under one protein result, with range 1 from 26 to 204, range 2 from 208 to 248, and range 3 from 202 to 227.

Answer 1

[Philipp Bayer]

These are three different reading frames as evidenced by +1, +2, and +3. Your given nucleotide input will be translated into all reading frames based on the start in the reference: +1, +2, +3, -1, -2, -3, and each reading frames can result in different protein blast hits, in your case you have three separate hits!