Is it possible to identify the strand of an RNA using UMI?

Question

[Carolina]

Hello to everyone who read this question! My research group did a high-throughput sRNA using the qiagen miRNA kit that uses UMIs, nothing in the kit says whether it is stranded or not, and from what I read in the literature sRNA kits are canonically strandless. My group is trying to recover the strand information from this RNA-seq. Is it possible somehow, would UMIs help with this? This image I attached is how the kit library was made. Does it have any indication of the conservation of strand information?

thank you very much for your attention.

[bk11]

You can probably use RSeQC tool. It works pretty well for RNASeq data. I have not tested it for microRNASeq data yet. However, I am expecting it should work. Please check with the infer_experiment.py function part.

Answer 1

[i.sudbery]

Well, from your image, it looks to me like the read with the UMI is the reverse read.