Running ASCAT without matched normal sample

Question

[Riccardo]

Hello!

I am trying to run ASCAT for Copy Number Analysis on tumour NGS-sequenced data. However, the samples do not have their matched normal. Is there a way to overcome this limitation and obtain a meaningful segmentation with ASCAT? I thought of creating a pooled reference germline sample. However, I think that this would lead to some problems, especially for the B-allele frequency signal. Thanks in advance!