How to find what is the most significantly modulated gene in GEO2R analysis?

Question

[guntul]

I performed a GEO2R analysis on the dataset GSE236761

GeneID  padj    pvalue  lfcSE   stat    log2FoldChange  baseMean    Symbol  Description
100506497   0.0000309   1.94e-09    0.391   -6.0027842  -2.34933389 13.57   CPVL-AS2    CPVL antisense RNA 2
9242    0.0004013   7.56e-08    0.407   5.3772388   2.18916521  37.51   MSC musculin
84649   0.0004013   5.11e-08    0.405   5.4474355   2.20725151  141.17  DGAT2   diacylglycerol O-acyltransferase 2
101929237   0.0008316   2.09e-07    0.42    -5.1911857  -2.18226839 17.86   LOC101929237    uncharacterized LOC101929237
4099    0.001678    5.27e-07    0.461   5.0161811   2.31151684  414.96  MAG myelin associated glycoprotein
55118   0.0020151   7.60e-07    0.366   4.9454853   1.81171282  351.17  CRTAC1  cartilage acidic protein 1
745 0.0034771   1.75e-06    0.458   4.7806231   2.18905791  84.12   MYRF    myelin regulatory factor
30812   0.0034771   1.53e-06    0.279   4.8071249   1.33943496  104.89  SOX8    SRY-box transcription factor 8
2537    0.0044323   2.51e-06    0.713   4.7076392   3.35635974  5752.59 IFI6    interferon alpha inducible protein 6

From this results, how can I find the most significantly modulated gene? Regards,

Answer 1

[ahmad mousavi]

You can selected genes with padj < 0.05 and log2FoldChange >= +1 for up regulated or log2FoldChange <= -1 for down regulated.

[guntul]

Thanks. If I have several rows after selection, which one can be said as the most modulated?

[bk11]

What do you mean by modulated here? If you are talking about the most differentially regulated, the most down-regulated gene should be the gene with lowest log2FC and padj values, and the most up-regulated gene should be the gene with the highest log2FC and lowest padj values.

[guntul]

Yes, that's what I mean. Thank you