Exception : [rapidjson internal assertion failure: IsObject()]
0
0
Entering edit mode
10 months ago
Andrea ▴ 10

I've installed Salmon and made the index, but once I run the analysis, I've got this error:

(salmon) andrea@Precision-7920-Tower:/hdd_a/andrea/Salmon$ sudo salmon quant -i GRCh38_salmon_index/ -l A -r /hdd_a/andrea/Salmon/Fastq/1_vehicle_R1.fastq.gz --validateMappings -o /Output/
### salmon (mapping-based) v0.12.0
### [ program ] => salmon 
### [ command ] => quant 
### [ index ] => { GRCh38_salmon_index/ }
### [ libType ] => { A }
### [ unmatedReads ] => { /hdd_a/andrea/Salmon/Fastq/1_vehicle_R1.fastq.gz }
### [ validateMappings ] => { }
### [ output ] => { /Output/ }
Logs will be written to /Output/logs
[2024-02-21 09:49:04.094] [jointLog] [info] Fragment incompatibility prior below threshold.  Incompatible fragments will be ignored.
[2024-02-21 09:49:04.094] [jointLog] [info] Usage of --validateMappings implies use of minScoreFraction. Since not explicitly specified, it is being set to 0.65
[2024-02-21 09:49:04.094] [jointLog] [info] Usage of --validateMappings implies use of range factorization. rangeFactorizationBins is being set to 4
[2024-02-21 09:49:04.094] [jointLog] [info] Usage of --validateMappings implies a default consensus slack of 1. Setting consensusSlack to 1.
[2024-02-21 09:49:04.094] [jointLog] [info] parsing read library format
[2024-02-21 09:49:04.094] [jointLog] [info] There is 1 library.
[2024-02-21 09:49:04.207] [jointLog] [info] Loading Quasi index
Exception : [rapidjson internal assertion failure: IsObject()]
salmon quant was invoked improperly.
For usage information, try salmon quant --help
Exiting.

Any suggestion? Thank you

salmon • 540 views
ADD COMMENT
0
Entering edit mode

sudo salmon

you should never run such tool as sudo

ADD REPLY
0
Entering edit mode

thank you, solved!

ADD REPLY

Login before adding your answer.

Traffic: 1693 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6